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HESI A2 Biology Practice Questions

15 free questions from the CinnaEntry HESI A2 biology bank (311 in the app). Tap an answer to check it. The rationale opens right away.

15 questions Rationale on every answer Updated October 17, 2026
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  1. Q1 / 15 Correct Not quite

    The basic unit of structure and function in all living things is the:

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    Answer: A. Cell

    Levels of organization: cell → tissue → organ → organ system → organism. The cell is the smallest unit that is itself alive.

  2. Q2 / 15 Correct Not quite

    The process of copying DNA into messenger RNA is called:

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    Answer: B. Transcription

    Transcription copies a DNA gene into mRNA in the nucleus; translation then builds the protein.

  3. Q3 / 15 Correct Not quite

    The greatest amount of available energy in a food chain is at the level of the:

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    Answer: B. Producers

    Producers hold the most energy; energy decreases at each higher level.

  4. Q4 / 15 Correct Not quite

    The final step of a scientific investigation is usually to:

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    Answer: B. Communicate the results

    After analyzing data and drawing conclusions, scientists communicate their results.

  5. Q5 / 15 Correct Not quite

    Plant cells have a rigid outer structure that animal cells lack, called the:

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    Answer: B. Cell wall

    Plant cells have a cell wall (of cellulose) for support; animal cells have only a cell membrane.

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  7. Q6 / 15 Correct Not quite

    A conclusion drawn from an experiment should be based on the:

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    Answer: B. Data and results

    Conclusions must be supported by the data collected, not opinion.

  8. Q7 / 15 Correct Not quite

    Active transport differs from passive transport because it:

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    Answer: A. Requires energy (ATP)

    Passive moves down the gradient for free; active moves UP the gradient and must pay in ATP. The sodium-potassium pump is the standard example.

  9. Q8 / 15 Correct Not quite

    Which term describes all the members of one species living in an area?

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    Answer: B. Population

    A population is all the individuals of a single species in one area.

  10. Q9 / 15 Correct Not quite

    The genetic information for making a specific protein is carried by a:

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    Answer: A. Gene

    Nesting order: nucleus contains chromosomes, chromosomes contain genes, genes code for proteins.

  11. Q10 / 15 Correct Not quite

    A cell that has a true, membrane-bound nucleus is called:

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    Answer: B. Eukaryotic

    Eukaryotic cells (plant, animal, fungi) contain a true nucleus enclosed by a membrane.

  12. Q11 / 15 Correct Not quite

    Beginning at Kingdom, which is the correct order from broadest to narrowest?

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    Answer: A. Kingdom, Phylum, Class, Order, Family, Genus, Species

    Kingdom, Phylum, Class, Order, Family, Genus, Species. Modern classification adds Domain above Kingdom as the broadest level of all, so the full sequence is Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species. Use any sentence whose initials match — inventing your own makes it stick.

  13. Q12 / 15 Correct Not quite

    During which phase of mitosis do chromosomes line up along the cell's equator?

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    Answer: A. Metaphase

    The first letters carry the meaning: Metaphase-Middle, Anaphase-Apart, Telophase-Two. That mnemonic fixes the whole sequence.

  14. Q13 / 15 Correct Not quite

    A species with very few members remaining is considered:

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    Answer: B. Endangered

    An endangered species is at risk of extinction due to low numbers.

  15. Q14 / 15 Correct Not quite

    The best everyday way to prevent the spread of germs is:

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    Answer: B. Washing your hands

    Regular handwashing is one of the most effective ways to reduce germ spread.

  16. Q15 / 15 Correct Not quite

    The jelly-like fluid that fills a cell and holds the organelles is the:

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    Answer: B. Cytoplasm

    Cytoplasm is the gel-like substance where the organelles are suspended and many reactions occur.

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